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Updated November 2019
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Protein Info
Short Name:
DOT1L
Full Name:
Histone-lysine N-methyltransferase, H3 lysine-79 specific
Alias:
DOT1; DOT1-like protein; DOT1-like, histone H3 methyltransferase; DOTL; H3-K79-HMTase; Histone H3-K79 methyltransferase; Histone methyltransferase DOT1L; KIAA1814; KMT4
Type:
EC 2.1.1.43; Amino Acid Metabolism - lysine degradation; Methyltransferase
Mass (Da):
184853
Number AA:
1739
UniProt ID:
Q8TEK3
International Prot ID:
IPI00170643
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005634
GO:0043226
GO:0043227
Uniprot
OncoNet
Molecular Function:
GO:0003677
GO:0018024
GO:0003824
PhosphoSite+
KinaseNET
Biological Process:
GO:0016568
GO:0006996
GO:0009987
Phosida
TranscriptoNet
STRING
Kinexus Products
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S11
K
L
E
L
R
L
K
S
P
V
G
A
E
P
A
Site 2
Y27
Y
P
W
P
L
P
V
Y
D
K
H
H
D
A
A
Site 3
T40
A
A
H
E
I
I
E
T
I
R
W
V
C
E
E
Site 4
Y63
E
N
Y
V
L
I
D
Y
D
T
K
S
F
E
S
Site 5
T65
Y
V
L
I
D
Y
D
T
K
S
F
E
S
M
Q
Site 6
S67
L
I
D
Y
D
T
K
S
F
E
S
M
Q
R
L
Site 7
S70
Y
D
T
K
S
F
E
S
M
Q
R
L
C
D
K
Site 8
Y78
M
Q
R
L
C
D
K
Y
N
R
A
I
D
S
I
Site 9
S84
K
Y
N
R
A
I
D
S
I
H
Q
L
W
K
G
Site 10
S103
M
K
L
N
T
R
P
S
T
G
L
L
R
H
I
Site 11
T104
K
L
N
T
R
P
S
T
G
L
L
R
H
I
L
Site 12
Y128
D
P
E
K
L
N
N
Y
E
P
F
S
P
E
V
Site 13
S132
L
N
N
Y
E
P
F
S
P
E
V
Y
G
E
T
Site 14
Y136
E
P
F
S
P
E
V
Y
G
E
T
S
F
D
L
Site 15
S140
P
E
V
Y
G
E
T
S
F
D
L
V
A
Q
M
Site 16
T154
M
I
D
E
I
K
M
T
D
D
D
L
F
V
D
Site 17
Y216
Y
G
K
K
H
A
E
Y
T
L
E
R
G
D
F
Site 18
T217
G
K
K
H
A
E
Y
T
L
E
R
G
D
F
L
Site 19
S225
L
E
R
G
D
F
L
S
E
E
W
R
E
R
I
Site 20
S268
K
E
G
G
R
I
V
S
S
K
P
F
A
P
L
Site 21
S269
E
G
G
R
I
V
S
S
K
P
F
A
P
L
N
Site 22
S281
P
L
N
F
R
I
N
S
R
N
L
S
D
I
G
Site 23
S285
R
I
N
S
R
N
L
S
D
I
G
T
I
M
R
Site 24
S297
I
M
R
V
V
E
L
S
P
L
K
G
S
V
S
Site 25
S302
E
L
S
P
L
K
G
S
V
S
W
T
G
K
P
Site 26
S304
S
P
L
K
G
S
V
S
W
T
G
K
P
V
S
Site 27
T306
L
K
G
S
V
S
W
T
G
K
P
V
S
Y
Y
Site 28
Y313
T
G
K
P
V
S
Y
Y
L
H
T
I
D
R
T
Site 29
Y325
D
R
T
I
L
E
N
Y
F
S
S
L
K
N
P
Site 30
S327
T
I
L
E
N
Y
F
S
S
L
K
N
P
K
L
Site 31
S349
R
R
R
Q
Q
R
E
S
K
S
N
A
A
T
P
Site 32
S351
R
Q
Q
R
E
S
K
S
N
A
A
T
P
T
K
Site 33
T355
E
S
K
S
N
A
A
T
P
T
K
G
P
E
G
Site 34
T357
K
S
N
A
A
T
P
T
K
G
P
E
G
K
V
Site 35
S374
P
A
D
A
P
M
D
S
G
A
E
E
E
K
A
Site 36
S390
A
A
T
V
K
K
P
S
P
S
K
A
R
K
K
Site 37
S392
T
V
K
K
P
S
P
S
K
A
R
K
K
K
L
Site 38
T419
G
R
P
K
K
M
N
T
A
N
P
E
R
K
P
Site 39
S442
A
L
H
A
Q
T
V
S
Q
T
A
A
S
S
P
Site 40
T444
H
A
Q
T
V
S
Q
T
A
A
S
S
P
Q
D
Site 41
S448
V
S
Q
T
A
A
S
S
P
Q
D
A
Y
R
S
Site 42
Y453
A
S
S
P
Q
D
A
Y
R
S
P
H
S
P
F
Site 43
S455
S
P
Q
D
A
Y
R
S
P
H
S
P
F
Y
Q
Site 44
S458
D
A
Y
R
S
P
H
S
P
F
Y
Q
L
P
P
Site 45
Y461
R
S
P
H
S
P
F
Y
Q
L
P
P
S
V
Q
Site 46
S466
P
F
Y
Q
L
P
P
S
V
Q
R
H
S
P
N
Site 47
S471
P
P
S
V
Q
R
H
S
P
N
P
L
L
V
A
Site 48
T480
N
P
L
L
V
A
P
T
P
P
A
L
Q
K
L
Site 49
S490
A
L
Q
K
L
L
E
S
F
K
I
Q
Y
L
Q
Site 50
T504
Q
F
L
A
Y
T
K
T
P
Q
Y
K
A
S
L
Site 51
Y507
A
Y
T
K
T
P
Q
Y
K
A
S
L
Q
E
L
Site 52
S510
K
T
P
Q
Y
K
A
S
L
Q
E
L
L
G
Q
Site 53
S534
G
A
A
Q
Q
L
L
S
H
C
Q
A
Q
K
E
Site 54
S572
I
Q
A
Q
K
E
I
S
A
H
N
Q
Q
L
R
Site 55
S582
N
Q
Q
L
R
E
Q
S
E
Q
L
E
Q
D
N
Site 56
S596
N
R
A
L
R
G
Q
S
L
Q
L
L
K
A
R
Site 57
S615
Q
L
D
W
A
T
L
S
L
E
K
L
L
K
E
Site 58
S628
K
E
K
Q
A
L
K
S
Q
I
S
E
K
Q
R
Site 59
S631
Q
A
L
K
S
Q
I
S
E
K
Q
R
H
C
L
Site 60
S650
S
I
V
E
L
E
K
S
Q
R
Q
Q
E
L
L
Site 61
S661
Q
E
L
L
Q
L
K
S
C
V
P
P
D
D
A
Site 62
S670
V
P
P
D
D
A
L
S
L
H
L
R
G
K
G
Site 63
S688
R
E
L
E
P
D
A
S
R
L
H
L
E
L
D
Site 64
S700
E
L
D
C
T
K
F
S
L
P
H
L
S
S
M
Site 65
S705
K
F
S
L
P
H
L
S
S
M
S
P
E
L
S
Site 66
S706
F
S
L
P
H
L
S
S
M
S
P
E
L
S
M
Site 67
S708
L
P
H
L
S
S
M
S
P
E
L
S
M
N
G
Site 68
S712
S
S
M
S
P
E
L
S
M
N
G
Q
A
A
G
Site 69
S727
Y
E
L
C
G
V
L
S
R
P
S
S
K
Q
N
Site 70
S730
C
G
V
L
S
R
P
S
S
K
Q
N
T
P
Q
Site 71
S731
G
V
L
S
R
P
S
S
K
Q
N
T
P
Q
Y
Site 72
T735
R
P
S
S
K
Q
N
T
P
Q
Y
L
A
S
P
Site 73
Y738
S
K
Q
N
T
P
Q
Y
L
A
S
P
L
D
Q
Site 74
S741
N
T
P
Q
Y
L
A
S
P
L
D
Q
E
V
V
Site 75
T751
D
Q
E
V
V
P
C
T
P
S
H
V
G
R
P
Site 76
S753
E
V
V
P
C
T
P
S
H
V
G
R
P
R
L
Site 77
S764
R
P
R
L
E
K
L
S
G
L
A
A
P
D
Y
Site 78
Y771
S
G
L
A
A
P
D
Y
T
R
L
S
P
A
K
Site 79
S775
A
P
D
Y
T
R
L
S
P
A
K
I
V
L
R
Site 80
S786
I
V
L
R
R
H
L
S
Q
D
H
T
V
P
G
Site 81
T790
R
H
L
S
Q
D
H
T
V
P
G
R
P
A
A
Site 82
S798
V
P
G
R
P
A
A
S
E
L
H
S
R
A
E
Site 83
S802
P
A
A
S
E
L
H
S
R
A
E
H
T
K
E
Site 84
Y814
T
K
E
N
G
L
P
Y
Q
S
P
S
V
P
G
Site 85
S816
E
N
G
L
P
Y
Q
S
P
S
V
P
G
S
M
Site 86
S818
G
L
P
Y
Q
S
P
S
V
P
G
S
M
K
L
Site 87
S822
Q
S
P
S
V
P
G
S
M
K
L
S
P
Q
D
Site 88
S826
V
P
G
S
M
K
L
S
P
Q
D
P
R
P
L
Site 89
S834
P
Q
D
P
R
P
L
S
P
G
A
L
Q
L
A
Site 90
S845
L
Q
L
A
G
E
K
S
S
E
K
G
L
R
E
Site 91
Y855
K
G
L
R
E
R
A
Y
G
S
S
G
E
L
I
Site 92
S857
L
R
E
R
A
Y
G
S
S
G
E
L
I
T
S
Site 93
S858
R
E
R
A
Y
G
S
S
G
E
L
I
T
S
L
Site 94
T873
P
I
S
I
P
L
S
T
V
Q
P
N
K
L
P
Site 95
S899
S
R
A
E
R
A
R
S
T
P
S
P
V
L
Q
Site 96
T900
R
A
E
R
A
R
S
T
P
S
P
V
L
Q
P
Site 97
S902
E
R
A
R
S
T
P
S
P
V
L
Q
P
R
D
Site 98
S911
V
L
Q
P
R
D
P
S
S
T
L
E
K
Q
I
Site 99
S912
L
Q
P
R
D
P
S
S
T
L
E
K
Q
I
G
Site 100
T913
Q
P
R
D
P
S
S
T
L
E
K
Q
I
G
A
Site 101
T957
A
G
S
P
A
S
L
T
P
G
A
E
P
A
T
Site 102
T964
T
P
G
A
E
P
A
T
L
D
E
S
S
S
S
Site 103
S968
E
P
A
T
L
D
E
S
S
S
S
G
S
L
F
Site 104
S969
P
A
T
L
D
E
S
S
S
S
G
S
L
F
A
Site 105
S970
A
T
L
D
E
S
S
S
S
G
S
L
F
A
T
Site 106
S971
T
L
D
E
S
S
S
S
G
S
L
F
A
T
V
Site 107
S973
D
E
S
S
S
S
G
S
L
F
A
T
V
G
S
Site 108
T977
S
S
G
S
L
F
A
T
V
G
S
R
S
S
T
Site 109
S980
S
L
F
A
T
V
G
S
R
S
S
T
P
Q
H
Site 110
S982
F
A
T
V
G
S
R
S
S
T
P
Q
H
P
L
Site 111
S983
A
T
V
G
S
R
S
S
T
P
Q
H
P
L
L
Site 112
T984
T
V
G
S
R
S
S
T
P
Q
H
P
L
L
L
Site 113
S997
L
L
A
Q
P
R
N
S
L
P
A
S
P
A
H
Site 114
S1001
P
R
N
S
L
P
A
S
P
A
H
Q
L
S
S
Site 115
S1007
A
S
P
A
H
Q
L
S
S
S
P
R
L
G
G
Site 116
S1008
S
P
A
H
Q
L
S
S
S
P
R
L
G
G
A
Site 117
S1009
P
A
H
Q
L
S
S
S
P
R
L
G
G
A
A
Site 118
S1024
Q
G
P
L
P
E
A
S
K
G
D
L
P
S
D
Site 119
S1030
A
S
K
G
D
L
P
S
D
S
G
F
S
D
P
Site 120
S1032
K
G
D
L
P
S
D
S
G
F
S
D
P
E
S
Site 121
S1035
L
P
S
D
S
G
F
S
D
P
E
S
E
A
K
Site 122
S1039
S
G
F
S
D
P
E
S
E
A
K
R
R
I
V
Site 123
S1055
T
I
T
T
G
A
G
S
A
K
Q
S
P
S
S
Site 124
S1059
G
A
G
S
A
K
Q
S
P
S
S
K
H
S
P
Site 125
S1061
G
S
A
K
Q
S
P
S
S
K
H
S
P
L
T
Site 126
S1062
S
A
K
Q
S
P
S
S
K
H
S
P
L
T
A
Site 127
S1065
Q
S
P
S
S
K
H
S
P
L
T
A
S
A
R
Site 128
T1068
S
S
K
H
S
P
L
T
A
S
A
R
G
D
C
Site 129
S1070
K
H
S
P
L
T
A
S
A
R
G
D
C
V
P
Site 130
S1083
V
P
S
H
G
Q
D
S
R
R
R
G
R
R
K
Site 131
S1093
R
G
R
R
K
R
A
S
A
G
T
P
S
L
S
Site 132
T1096
R
K
R
A
S
A
G
T
P
S
L
S
A
G
V
Site 133
S1098
R
A
S
A
G
T
P
S
L
S
A
G
V
S
P
Site 134
S1100
S
A
G
T
P
S
L
S
A
G
V
S
P
K
R
Site 135
S1104
P
S
L
S
A
G
V
S
P
K
R
R
A
L
P
Site 136
S1112
P
K
R
R
A
L
P
S
V
A
G
L
F
T
Q
Site 137
S1121
A
G
L
F
T
Q
P
S
G
S
P
L
N
L
N
Site 138
S1123
L
F
T
Q
P
S
G
S
P
L
N
L
N
S
M
Site 139
S1129
G
S
P
L
N
L
N
S
M
V
S
N
I
N
Q
Site 140
T1141
I
N
Q
P
L
E
I
T
A
I
S
S
P
E
T
Site 141
S1145
L
E
I
T
A
I
S
S
P
E
T
S
L
K
S
Site 142
T1148
T
A
I
S
S
P
E
T
S
L
K
S
S
P
V
Site 143
S1149
A
I
S
S
P
E
T
S
L
K
S
S
P
V
P
Site 144
S1152
S
P
E
T
S
L
K
S
S
P
V
P
Y
Q
D
Site 145
S1153
P
E
T
S
L
K
S
S
P
V
P
Y
Q
D
H
Site 146
Y1157
L
K
S
S
P
V
P
Y
Q
D
H
D
Q
P
P
Site 147
S1173
L
K
K
E
R
P
L
S
Q
T
N
G
A
H
Y
Site 148
T1175
K
E
R
P
L
S
Q
T
N
G
A
H
Y
S
P
Site 149
S1181
Q
T
N
G
A
H
Y
S
P
L
T
S
D
E
E
Site 150
T1184
G
A
H
Y
S
P
L
T
S
D
E
E
P
G
S
Site 151
S1185
A
H
Y
S
P
L
T
S
D
E
E
P
G
S
E
Site 152
S1191
T
S
D
E
E
P
G
S
E
D
E
P
S
S
A
Site 153
S1196
P
G
S
E
D
E
P
S
S
A
R
I
E
R
K
Site 154
S1197
G
S
E
D
E
P
S
S
A
R
I
E
R
K
I
Site 155
T1206
R
I
E
R
K
I
A
T
I
S
L
E
S
K
S
Site 156
S1208
E
R
K
I
A
T
I
S
L
E
S
K
S
P
P
Site 157
S1213
T
I
S
L
E
S
K
S
P
P
K
T
L
E
N
Site 158
T1217
E
S
K
S
P
P
K
T
L
E
N
G
G
G
L
Site 159
S1238
P
A
G
E
P
V
N
S
S
K
W
K
S
T
F
Site 160
S1239
A
G
E
P
V
N
S
S
K
W
K
S
T
F
S
Site 161
S1243
V
N
S
S
K
W
K
S
T
F
S
P
I
S
D
Site 162
T1244
N
S
S
K
W
K
S
T
F
S
P
I
S
D
I
Site 163
S1246
S
K
W
K
S
T
F
S
P
I
S
D
I
G
L
Site 164
S1249
K
S
T
F
S
P
I
S
D
I
G
L
A
K
S
Site 165
S1256
S
D
I
G
L
A
K
S
A
D
S
P
L
Q
A
Site 166
S1259
G
L
A
K
S
A
D
S
P
L
Q
A
S
S
A
Site 167
S1264
A
D
S
P
L
Q
A
S
S
A
L
S
Q
N
S
Site 168
S1265
D
S
P
L
Q
A
S
S
A
L
S
Q
N
S
L
Site 169
S1271
S
S
A
L
S
Q
N
S
L
F
T
F
R
P
A
Site 170
T1274
L
S
Q
N
S
L
F
T
F
R
P
A
L
E
E
Site 171
S1283
R
P
A
L
E
E
P
S
A
D
A
K
L
A
A
Site 172
S1299
P
R
K
G
F
P
G
S
L
S
G
A
D
G
L
Site 173
S1301
K
G
F
P
G
S
L
S
G
A
D
G
L
S
P
Site 174
S1307
L
S
G
A
D
G
L
S
P
G
T
N
P
A
N
Site 175
S1332
D
L
S
L
H
S
F
S
D
G
A
S
L
P
H
Site 176
S1336
H
S
F
S
D
G
A
S
L
P
H
K
G
P
E
Site 177
S1348
G
P
E
A
A
G
L
S
S
P
L
S
F
P
S
Site 178
S1349
P
E
A
A
G
L
S
S
P
L
S
F
P
S
Q
Site 179
S1352
A
G
L
S
S
P
L
S
F
P
S
Q
R
G
K
Site 180
S1355
S
S
P
L
S
F
P
S
Q
R
G
K
E
G
S
Site 181
S1362
S
Q
R
G
K
E
G
S
D
A
N
P
F
L
S
Site 182
S1369
S
D
A
N
P
F
L
S
K
R
Q
L
D
G
L
Site 183
S1384
A
G
L
K
G
E
G
S
R
G
K
E
A
G
E
Site 184
T1403
L
C
G
P
T
D
K
T
P
L
L
S
G
K
A
Site 185
S1456
S
S
A
G
G
A
A
S
S
A
Q
T
H
R
S
Site 186
S1457
S
A
G
G
A
A
S
S
A
Q
T
H
R
S
F
Site 187
T1460
G
A
A
S
S
A
Q
T
H
R
S
F
L
G
P
Site 188
S1463
S
S
A
Q
T
H
R
S
F
L
G
P
F
P
P
Site 189
T1515
V
H
V
S
S
A
A
T
R
L
T
N
S
H
A
Site 190
T1518
S
S
A
A
T
R
L
T
N
S
H
A
M
G
S
Site 191
S1520
A
A
T
R
L
T
N
S
H
A
M
G
S
F
S
Site 192
S1525
T
N
S
H
A
M
G
S
F
S
G
V
A
G
G
Site 193
S1547
N
H
A
V
P
S
A
S
A
H
P
F
G
A
R
Site 194
S1563
G
R
G
A
A
C
G
S
A
T
L
G
P
S
P
Site 195
S1576
S
P
L
Q
A
A
A
S
A
S
A
S
S
F
Q
Site 196
S1587
S
S
F
Q
A
P
A
S
V
E
T
R
P
P
P
Site 197
S1612
P
P
A
H
L
G
R
S
P
A
G
P
P
V
L
Site 198
T1634
A
A
L
P
P
P
P
T
L
L
A
S
N
P
E
Site 199
S1647
P
E
P
A
L
L
Q
S
L
A
S
L
P
P
N
Site 200
T1661
N
Q
A
F
L
P
P
T
S
A
A
S
L
P
P
Site 201
S1665
L
P
P
T
S
A
A
S
L
P
P
A
N
A
S
Site 202
S1679
S
L
S
I
K
L
T
S
L
P
H
K
G
A
R
Site 203
S1688
P
H
K
G
A
R
P
S
F
T
V
H
H
Q
P
Site 204
T1690
K
G
A
R
P
S
F
T
V
H
H
Q
P
L
P
Site 205
S1734
P
P
Y
A
A
H
L
S
G
V
K
P
R
_
_
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation