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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
RFFL
Full Name:
E3 ubiquitin-protein ligase rififylin
Alias:
E3 ubiquitin-protein ligase rififylin: RING finger and FYVE-like domain-containing protein 1: FYVE-RING finger protein Sakura: Caspases-8 and -10-associated RING finger protein 2: Caspase regulator CARP2: RING finger protein 189: RING finger protein 34-like: E3 ubiquitin-protein ligase rififylin: RING finger and FYVE-like domain-containing protein 1: FYVE-RING finger protein Sakura: Caspases-8 and -10-associated RING finger protein 2: Caspase regulator CARP2: RING finger protein 189: RING finger protein 34-like; Fring; Hypothetical protein FLJ35793; Rififylin; Ring finger and FYVE-like domain containing 1; RNF189; RNF34L
Type:
Ubiquitin ligase; Vesicle protein; Ubiquitin conjugating system; EC 6.3.2.-; Ligase
Mass (Da):
40514
Number AA:
363
UniProt ID:
Q8WZ73
International Prot ID:
IPI00103867
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0019898
Uniprot
OncoNet
Molecular Function:
GO:0016874
GO:0005515
GO:0008270
PhosphoSite+
KinaseNET
Biological Process:
GO:0006915
GO:0019941
Phosida
TranscriptoNet
STRING
Kinexus Products
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
Y29
Q
G
A
R
M
Q
A
Y
S
N
P
G
Y
S
S
Site 2
S30
G
A
R
M
Q
A
Y
S
N
P
G
Y
S
S
F
Site 3
Y34
Q
A
Y
S
N
P
G
Y
S
S
F
P
S
P
T
Site 4
S35
A
Y
S
N
P
G
Y
S
S
F
P
S
P
T
G
Site 5
S36
Y
S
N
P
G
Y
S
S
F
P
S
P
T
G
L
Site 6
S39
P
G
Y
S
S
F
P
S
P
T
G
L
E
P
S
Site 7
S46
S
P
T
G
L
E
P
S
C
K
S
C
G
A
H
Site 8
S49
G
L
E
P
S
C
K
S
C
G
A
H
F
A
N
Site 9
T62
A
N
T
A
R
K
Q
T
C
L
D
C
K
K
N
Site 10
T94
L
C
Q
R
F
R
A
T
A
F
Q
R
E
E
L
Site 11
Y112
K
V
K
D
L
R
D
Y
L
S
L
H
D
I
S
Site 12
S114
K
D
L
R
D
Y
L
S
L
H
D
I
S
T
E
Site 13
S119
Y
L
S
L
H
D
I
S
T
E
M
C
R
E
K
Site 14
T120
L
S
L
H
D
I
S
T
E
M
C
R
E
K
E
Site 15
S141
L
G
Q
Q
P
V
I
S
Q
E
D
R
T
R
A
Site 16
T146
V
I
S
Q
E
D
R
T
R
A
S
T
L
S
P
Site 17
S149
Q
E
D
R
T
R
A
S
T
L
S
P
D
F
P
Site 18
T150
E
D
R
T
R
A
S
T
L
S
P
D
F
P
E
Site 19
S152
R
T
R
A
S
T
L
S
P
D
F
P
E
Q
Q
Site 20
T163
P
E
Q
Q
A
F
L
T
Q
P
H
S
S
M
V
Site 21
S167
A
F
L
T
Q
P
H
S
S
M
V
P
P
T
S
Site 22
S168
F
L
T
Q
P
H
S
S
M
V
P
P
T
S
P
Site 23
T173
H
S
S
M
V
P
P
T
S
P
N
L
P
S
S
Site 24
S174
S
S
M
V
P
P
T
S
P
N
L
P
S
S
S
Site 25
S179
P
T
S
P
N
L
P
S
S
S
A
Q
A
T
S
Site 26
S180
T
S
P
N
L
P
S
S
S
A
Q
A
T
S
V
Site 27
S181
S
P
N
L
P
S
S
S
A
Q
A
T
S
V
P
Site 28
T185
P
S
S
S
A
Q
A
T
S
V
P
P
A
Q
V
Site 29
S186
S
S
S
A
Q
A
T
S
V
P
P
A
Q
V
Q
Site 30
S203
Q
Q
A
N
G
H
V
S
Q
D
Q
E
E
P
V
Site 31
Y211
Q
D
Q
E
E
P
V
Y
L
E
S
V
A
R
V
Site 32
S214
E
E
P
V
Y
L
E
S
V
A
R
V
P
A
E
Site 33
T224
R
V
P
A
E
D
E
T
Q
S
I
D
S
E
D
Site 34
S226
P
A
E
D
E
T
Q
S
I
D
S
E
D
S
F
Site 35
S229
D
E
T
Q
S
I
D
S
E
D
S
F
V
P
G
Site 36
S232
Q
S
I
D
S
E
D
S
F
V
P
G
R
R
A
Site 37
S240
F
V
P
G
R
R
A
S
L
S
D
L
T
D
L
Site 38
S242
P
G
R
R
A
S
L
S
D
L
T
D
L
E
D
Site 39
T245
R
A
S
L
S
D
L
T
D
L
E
D
I
E
G
Site 40
T254
L
E
D
I
E
G
L
T
V
R
Q
L
K
E
I
Site 41
Y269
L
A
R
N
F
V
N
Y
K
G
C
C
E
K
W
Site 42
T283
W
E
L
M
E
R
V
T
R
L
Y
K
D
Q
K
Site 43
Y286
M
E
R
V
T
R
L
Y
K
D
Q
K
G
L
Q
Site 44
S297
K
G
L
Q
H
L
V
S
G
A
E
D
Q
N
G
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation