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Updated November 2019
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Protein Info
Short Name:
ROBO4
Full Name:
Roundabout homolog 4
Alias:
Magic roundabout
Type:
Mass (Da):
107457
Number AA:
1007
UniProt ID:
Q8WZ75
International Prot ID:
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
Uniprot
OncoNet
Molecular Function:
PhosphoSite+
KinaseNET
Biological Process:
Phosida
TranscriptoNet
STRING
Kinexus Products
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S3
_
_
_
_
_
M
G
S
G
G
D
S
L
L
G
Site 2
S7
_
M
G
S
G
G
D
S
L
L
G
G
R
G
S
Site 3
S30
M
G
G
M
A
Q
D
S
P
P
Q
I
L
V
H
Site 4
S52
G
P
G
P
A
R
M
S
C
Q
A
S
G
Q
P
Site 5
S56
A
R
M
S
C
Q
A
S
G
Q
P
P
P
T
I
Site 6
T62
A
S
G
Q
P
P
P
T
I
R
W
L
L
N
G
Site 7
S73
L
L
N
G
Q
P
L
S
M
V
P
P
D
P
H
Site 8
S106
A
H
D
G
Q
A
L
S
T
D
L
G
V
Y
T
Site 9
Y112
L
S
T
D
L
G
V
Y
T
C
E
A
S
N
R
Site 10
T113
S
T
D
L
G
V
Y
T
C
E
A
S
N
R
L
Site 11
T122
E
A
S
N
R
L
G
T
A
V
S
R
G
A
R
Site 12
S125
N
R
L
G
T
A
V
S
R
G
A
R
L
S
V
Site 13
S131
V
S
R
G
A
R
L
S
V
A
V
L
R
E
D
Site 14
T186
A
L
Q
P
G
R
H
T
V
S
G
G
S
L
L
Site 15
S188
Q
P
G
R
H
T
V
S
G
G
S
L
L
M
A
Site 16
S200
L
M
A
R
A
E
K
S
D
E
G
T
Y
M
C
Site 17
T204
A
E
K
S
D
E
G
T
Y
M
C
V
A
T
N
Site 18
Y205
E
K
S
D
E
G
T
Y
M
C
V
A
T
N
S
Site 19
S218
N
S
A
G
H
R
E
S
R
A
A
R
V
S
I
Site 20
S224
E
S
R
A
A
R
V
S
I
Q
E
P
Q
D
Y
Site 21
Y231
S
I
Q
E
P
Q
D
Y
T
E
P
V
E
L
L
Site 22
T232
I
Q
E
P
Q
D
Y
T
E
P
V
E
L
L
A
Site 23
S267
P
R
P
A
V
W
L
S
W
K
V
S
G
P
A
Site 24
T288
T
A
L
F
R
T
Q
T
A
P
G
G
Q
G
A
Site 25
Y319
G
L
H
W
G
Q
D
Y
E
F
K
V
R
P
S
Site 26
S326
Y
E
F
K
V
R
P
S
S
G
R
A
R
G
P
Site 27
S327
E
F
K
V
R
P
S
S
G
R
A
R
G
P
D
Site 28
S335
G
R
A
R
G
P
D
S
N
V
L
L
L
R
L
Site 29
S348
R
L
P
E
K
V
P
S
A
P
P
Q
E
V
T
Site 30
T355
S
A
P
P
Q
E
V
T
L
K
P
G
N
G
T
Site 31
Y382
H
N
G
I
I
R
G
Y
Q
V
W
S
L
G
N
Site 32
S386
I
R
G
Y
Q
V
W
S
L
G
N
T
S
L
P
Site 33
T390
Q
V
W
S
L
G
N
T
S
L
P
P
A
N
W
Site 34
S391
V
W
S
L
G
N
T
S
L
P
P
A
N
W
T
Site 35
T410
Q
T
Q
L
E
I
A
T
H
M
P
G
S
Y
C
Site 36
T447
E
Q
A
M
E
R
A
T
Q
E
P
S
E
H
G
Site 37
T457
P
S
E
H
G
P
W
T
L
E
Q
L
R
A
T
Site 38
T464
T
L
E
Q
L
R
A
T
L
K
R
P
E
V
I
Site 39
Y504
V
H
L
G
P
G
L
Y
R
Y
T
S
E
D
A
Site 40
Y506
L
G
P
G
L
Y
R
Y
T
S
E
D
A
I
L
Site 41
T507
G
P
G
L
Y
R
Y
T
S
E
D
A
I
L
K
Site 42
S508
P
G
L
Y
R
Y
T
S
E
D
A
I
L
K
H
Site 43
S520
L
K
H
R
M
D
H
S
D
S
Q
W
L
A
D
Site 44
T528
D
S
Q
W
L
A
D
T
W
R
S
T
S
G
S
Site 45
S531
W
L
A
D
T
W
R
S
T
S
G
S
R
D
L
Site 46
T532
L
A
D
T
W
R
S
T
S
G
S
R
D
L
S
Site 47
S533
A
D
T
W
R
S
T
S
G
S
R
D
L
S
S
Site 48
S535
T
W
R
S
T
S
G
S
R
D
L
S
S
S
S
Site 49
S539
T
S
G
S
R
D
L
S
S
S
S
S
L
S
S
Site 50
S540
S
G
S
R
D
L
S
S
S
S
S
L
S
S
R
Site 51
S541
G
S
R
D
L
S
S
S
S
S
L
S
S
R
L
Site 52
S542
S
R
D
L
S
S
S
S
S
L
S
S
R
L
G
Site 53
S543
R
D
L
S
S
S
S
S
L
S
S
R
L
G
A
Site 54
S545
L
S
S
S
S
S
L
S
S
R
L
G
A
D
A
Site 55
S546
S
S
S
S
S
L
S
S
R
L
G
A
D
A
R
Site 56
S561
D
P
L
D
C
R
R
S
L
L
S
W
D
S
R
Site 57
S564
D
C
R
R
S
L
L
S
W
D
S
R
S
P
G
Site 58
S567
R
S
L
L
S
W
D
S
R
S
P
G
V
P
L
Site 59
S569
L
L
S
W
D
S
R
S
P
G
V
P
L
L
P
Site 60
S584
D
T
S
T
F
Y
G
S
L
I
A
E
L
P
S
Site 61
S592
L
I
A
E
L
P
S
S
T
P
A
R
P
S
P
Site 62
T593
I
A
E
L
P
S
S
T
P
A
R
P
S
P
Q
Site 63
S598
S
S
T
P
A
R
P
S
P
Q
V
P
A
V
R
Site 64
S615
P
P
Q
L
A
Q
L
S
S
P
C
S
S
S
D
Site 65
S616
P
Q
L
A
Q
L
S
S
P
C
S
S
S
D
S
Site 66
S619
A
Q
L
S
S
P
C
S
S
S
D
S
L
C
S
Site 67
S620
Q
L
S
S
P
C
S
S
S
D
S
L
C
S
R
Site 68
S621
L
S
S
P
C
S
S
S
D
S
L
C
S
R
R
Site 69
S623
S
P
C
S
S
S
D
S
L
C
S
R
R
G
L
Site 70
S626
S
S
S
D
S
L
C
S
R
R
G
L
S
S
P
Site 71
S631
L
C
S
R
R
G
L
S
S
P
R
L
S
L
A
Site 72
S632
C
S
R
R
G
L
S
S
P
R
L
S
L
A
P
Site 73
S636
G
L
S
S
P
R
L
S
L
A
P
A
E
A
W
Site 74
S656
Q
E
L
Q
H
A
N
S
S
P
L
L
R
G
S
Site 75
S657
E
L
Q
H
A
N
S
S
P
L
L
R
G
S
H
Site 76
S663
S
S
P
L
L
R
G
S
H
S
L
E
L
R
A
Site 77
S665
P
L
L
R
G
S
H
S
L
E
L
R
A
C
E
Site 78
S678
C
E
L
G
N
R
G
S
K
N
L
S
Q
S
P
Site 79
S682
N
R
G
S
K
N
L
S
Q
S
P
G
A
V
P
Site 80
S684
G
S
K
N
L
S
Q
S
P
G
A
V
P
Q
A
Site 81
S706
G
P
K
L
L
S
S
S
N
E
L
V
T
R
H
Site 82
T711
S
S
S
N
E
L
V
T
R
H
L
P
P
A
P
Site 83
T724
A
P
L
F
P
H
E
T
P
P
T
Q
S
Q
Q
Site 84
T727
F
P
H
E
T
P
P
T
Q
S
Q
Q
T
Q
P
Site 85
S729
H
E
T
P
P
T
Q
S
Q
Q
T
Q
P
P
V
Site 86
S758
I
P
I
L
S
P
C
S
P
P
S
P
Q
A
S
Site 87
S761
L
S
P
C
S
P
P
S
P
Q
A
S
S
L
S
Site 88
S765
S
P
P
S
P
Q
A
S
S
L
S
G
P
S
P
Site 89
S766
P
P
S
P
Q
A
S
S
L
S
G
P
S
P
A
Site 90
S768
S
P
Q
A
S
S
L
S
G
P
S
P
A
S
S
Site 91
S771
A
S
S
L
S
G
P
S
P
A
S
S
R
L
S
Site 92
S774
L
S
G
P
S
P
A
S
S
R
L
S
S
S
S
Site 93
S775
S
G
P
S
P
A
S
S
R
L
S
S
S
S
L
Site 94
S778
S
P
A
S
S
R
L
S
S
S
S
L
S
S
L
Site 95
S779
P
A
S
S
R
L
S
S
S
S
L
S
S
L
G
Site 96
S780
A
S
S
R
L
S
S
S
S
L
S
S
L
G
E
Site 97
S781
S
S
R
L
S
S
S
S
L
S
S
L
G
E
D
Site 98
S783
R
L
S
S
S
S
L
S
S
L
G
E
D
Q
D
Site 99
S784
L
S
S
S
S
L
S
S
L
G
E
D
Q
D
S
Site 100
S791
S
L
G
E
D
Q
D
S
V
L
T
P
E
E
V
Site 101
T794
E
D
Q
D
S
V
L
T
P
E
E
V
A
L
C
Site 102
S805
V
A
L
C
L
E
L
S
E
G
E
E
T
P
R
Site 103
T810
E
L
S
E
G
E
E
T
P
R
N
S
V
S
P
Site 104
S814
G
E
E
T
P
R
N
S
V
S
P
M
P
R
A
Site 105
S816
E
T
P
R
N
S
V
S
P
M
P
R
A
P
S
Site 106
S823
S
P
M
P
R
A
P
S
P
P
T
T
Y
G
Y
Site 107
T826
P
R
A
P
S
P
P
T
T
Y
G
Y
I
S
V
Site 108
T827
R
A
P
S
P
P
T
T
Y
G
Y
I
S
V
P
Site 109
Y828
A
P
S
P
P
T
T
Y
G
Y
I
S
V
P
T
Site 110
Y830
S
P
P
T
T
Y
G
Y
I
S
V
P
T
A
S
Site 111
S832
P
T
T
Y
G
Y
I
S
V
P
T
A
S
E
F
Site 112
T865
C
P
P
R
P
C
L
T
P
T
P
S
E
G
S
Site 113
T867
P
R
P
C
L
T
P
T
P
S
E
G
S
L
A
Site 114
S869
P
C
L
T
P
T
P
S
E
G
S
L
A
N
G
Site 115
S872
T
P
T
P
S
E
G
S
L
A
N
G
W
G
S
Site 116
S881
A
N
G
W
G
S
A
S
E
D
N
A
A
S
A
Site 117
S887
A
S
E
D
N
A
A
S
A
R
A
S
L
V
S
Site 118
S891
N
A
A
S
A
R
A
S
L
V
S
S
S
D
G
Site 119
S894
S
A
R
A
S
L
V
S
S
S
D
G
S
F
L
Site 120
S895
A
R
A
S
L
V
S
S
S
D
G
S
F
L
A
Site 121
S896
R
A
S
L
V
S
S
S
D
G
S
F
L
A
D
Site 122
S934
D
C
V
F
I
D
A
S
S
P
P
S
P
R
D
Site 123
S935
C
V
F
I
D
A
S
S
P
P
S
P
R
D
E
Site 124
S938
I
D
A
S
S
P
P
S
P
R
D
E
I
F
L
Site 125
T946
P
R
D
E
I
F
L
T
P
N
L
S
L
P
L
Site 126
T969
E
D
M
E
V
S
H
T
Q
R
L
G
R
G
M
Site 127
S983
M
P
P
W
P
P
D
S
Q
I
S
S
Q
R
S
Site 128
S986
W
P
P
D
S
Q
I
S
S
Q
R
S
Q
L
H
Site 129
S987
P
P
D
S
Q
I
S
S
Q
R
S
Q
L
H
C
Site 130
S990
S
Q
I
S
S
Q
R
S
Q
L
H
C
R
M
P
Site 131
S1002
R
M
P
K
A
G
A
S
P
V
D
Y
S
_
_
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation