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Updated November 2019
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Protein Info
Short Name:
GBF1
Full Name:
Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
Alias:
ARF1GEF; BFA-resistant GEF 1; Golgi-specific brefeldin A resistant guanine nucleotide exchange factor 1; KIAA0248
Type:
Guanine nucleotide exchange factor, ARF
Mass (Da):
206446
Number AA:
1859
UniProt ID:
Q92538
International Prot ID:
IPI00021954
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0000139
GO:0005622
GO:0005794
Uniprot
OncoNet
Molecular Function:
GO:0005086
GO:0005515
GO:0005086
PhosphoSite+
KinaseNET
Biological Process:
GO:0048205
GO:0006892
GO:0032012
Phosida
TranscriptoNet
STRING
Kinexus Products
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S27
I
K
R
N
A
R
W
S
T
H
T
P
L
D
E
Site 2
T28
K
R
N
A
R
W
S
T
H
T
P
L
D
E
E
Site 3
T30
N
A
R
W
S
T
H
T
P
L
D
E
E
R
D
Site 4
S42
E
R
D
P
L
L
H
S
F
G
H
L
K
E
V
Site 5
S52
H
L
K
E
V
L
N
S
I
T
E
L
S
E
I
Site 6
S74
P
F
L
E
V
I
R
S
E
D
T
T
G
P
I
Site 7
T77
E
V
I
R
S
E
D
T
T
G
P
I
T
G
L
Site 8
T87
P
I
T
G
L
A
L
T
S
V
N
K
F
L
S
Site 9
T101
S
Y
A
L
I
D
P
T
H
E
G
T
A
E
G
Site 10
T117
E
N
M
A
D
A
V
T
H
A
R
F
V
G
T
Site 11
S156
G
A
H
L
T
N
E
S
V
C
E
I
M
Q
S
Site 12
S174
I
C
F
E
M
R
L
S
E
L
L
R
K
S
A
Site 13
S180
L
S
E
L
L
R
K
S
A
E
H
T
L
V
D
Site 14
T184
L
R
K
S
A
E
H
T
L
V
D
M
V
Q
L
Site 15
Y206
F
K
E
E
P
K
N
Y
V
G
T
N
M
K
K
Site 16
S222
K
M
R
A
G
G
M
S
D
S
S
K
W
K
K
Site 17
S224
R
A
G
G
M
S
D
S
S
K
W
K
K
Q
K
Site 18
S225
A
G
G
M
S
D
S
S
K
W
K
K
Q
K
R
Site 19
S233
K
W
K
K
Q
K
R
S
P
R
P
P
R
H
M
Site 20
T241
P
R
P
P
R
H
M
T
K
V
T
P
G
S
E
Site 21
T244
P
R
H
M
T
K
V
T
P
G
S
E
L
P
T
Site 22
S247
M
T
K
V
T
P
G
S
E
L
P
T
P
N
G
Site 23
T251
T
P
G
S
E
L
P
T
P
N
G
T
T
L
S
Site 24
T256
L
P
T
P
N
G
T
T
L
S
S
N
L
T
G
Site 25
S258
T
P
N
G
T
T
L
S
S
N
L
T
G
G
M
Site 26
S259
P
N
G
T
T
L
S
S
N
L
T
G
G
M
P
Site 27
S276
D
V
P
T
P
I
S
S
A
S
S
E
A
A
S
Site 28
S278
P
T
P
I
S
S
A
S
S
E
A
A
S
A
V
Site 29
S283
S
A
S
S
E
A
A
S
A
V
V
S
P
S
T
Site 30
T290
S
A
V
V
S
P
S
T
D
S
G
L
E
F
S
Site 31
S292
V
V
S
P
S
T
D
S
G
L
E
F
S
S
Q
Site 32
S298
D
S
G
L
E
F
S
S
Q
T
T
S
K
E
D
Site 33
S302
E
F
S
S
Q
T
T
S
K
E
D
L
T
D
L
Site 34
T307
T
T
S
K
E
D
L
T
D
L
E
Q
P
G
S
Site 35
S314
T
D
L
E
Q
P
G
S
P
G
Y
S
T
A
T
Site 36
S318
Q
P
G
S
P
G
Y
S
T
A
T
E
P
G
S
Site 37
T319
P
G
S
P
G
Y
S
T
A
T
E
P
G
S
S
Site 38
T321
S
P
G
Y
S
T
A
T
E
P
G
S
S
E
L
Site 39
S325
S
T
A
T
E
P
G
S
S
E
L
G
V
P
E
Site 40
S326
T
A
T
E
P
G
S
S
E
L
G
V
P
E
Q
Site 41
S345
E
G
T
H
V
E
K
S
Q
S
A
S
V
E
S
Site 42
S347
T
H
V
E
K
S
Q
S
A
S
V
E
S
I
P
Site 43
S349
V
E
K
S
Q
S
A
S
V
E
S
I
P
E
V
Site 44
S362
E
V
L
E
E
C
T
S
P
A
D
H
S
D
S
Site 45
S367
C
T
S
P
A
D
H
S
D
S
A
S
V
H
D
Site 46
S369
S
P
A
D
H
S
D
S
A
S
V
H
D
M
D
Site 47
S371
A
D
H
S
D
S
A
S
V
H
D
M
D
Y
V
Site 48
Y377
A
S
V
H
D
M
D
Y
V
N
P
R
G
V
R
Site 49
T386
N
P
R
G
V
R
F
T
Q
S
S
Q
K
E
G
Site 50
S388
R
G
V
R
F
T
Q
S
S
Q
K
E
G
T
A
Site 51
S389
G
V
R
F
T
Q
S
S
Q
K
E
G
T
A
L
Site 52
T415
F
R
F
L
I
S
L
T
N
P
H
D
R
H
N
Site 53
T507
K
K
L
M
E
I
I
T
V
E
N
P
K
M
P
Site 54
Y515
V
E
N
P
K
M
P
Y
E
M
K
E
M
A
L
Site 55
Y539
P
S
F
V
T
E
L
Y
I
N
Y
D
C
D
Y
Site 56
Y542
V
T
E
L
Y
I
N
Y
D
C
D
Y
Y
C
S
Site 57
Y546
Y
I
N
Y
D
C
D
Y
Y
C
S
N
L
F
E
Site 58
Y547
I
N
Y
D
C
D
Y
Y
C
S
N
L
F
E
E
Site 59
T556
S
N
L
F
E
E
L
T
K
L
L
S
K
N
A
Site 60
S560
E
E
L
T
K
L
L
S
K
N
A
F
P
V
S
Site 61
S567
S
K
N
A
F
P
V
S
G
Q
L
Y
T
T
H
Site 62
S599
C
Q
A
K
V
L
N
S
L
T
Q
Q
E
K
K
Site 63
T601
A
K
V
L
N
S
L
T
Q
Q
E
K
K
E
T
Site 64
T608
T
Q
Q
E
K
K
E
T
A
R
P
S
C
E
I
Site 65
S612
K
K
E
T
A
R
P
S
C
E
I
V
D
G
T
Site 66
T619
S
C
E
I
V
D
G
T
R
E
A
S
N
T
E
Site 67
S623
V
D
G
T
R
E
A
S
N
T
E
R
T
A
S
Site 68
T625
G
T
R
E
A
S
N
T
E
R
T
A
S
D
G
Site 69
S630
S
N
T
E
R
T
A
S
D
G
K
A
V
G
M
Site 70
S659
L
P
P
E
H
G
K
S
G
C
S
D
L
E
E
Site 71
S662
E
H
G
K
S
G
C
S
D
L
E
E
A
V
D
Site 72
S670
D
L
E
E
A
V
D
S
G
A
D
K
K
F
A
Site 73
S684
A
R
K
P
P
R
F
S
C
L
L
P
D
P
R
Site 74
T734
L
T
I
P
M
D
N
T
E
V
A
Q
W
L
R
Site 75
S756
K
M
I
G
E
F
V
S
D
R
K
N
I
D
L
Site 76
S772
E
S
F
V
S
T
F
S
F
Q
G
L
R
L
D
Site 77
S820
N
G
S
P
F
A
N
S
D
A
C
F
S
L
A
Site 78
T850
R
K
Q
N
A
P
M
T
L
E
E
F
R
K
N
Site 79
Y876
Q
D
I
L
E
D
M
Y
H
A
I
K
N
E
E
Site 80
S945
L
S
Y
V
F
D
K
S
L
E
E
T
I
I
Q
Site 81
T949
F
D
K
S
L
E
E
T
I
I
Q
K
A
I
S
Site 82
S989
C
K
F
T
A
L
S
S
E
S
I
E
N
L
P
Site 83
S997
E
S
I
E
N
L
P
S
V
F
G
S
N
P
K
Site 84
S1001
N
L
P
S
V
F
G
S
N
P
K
A
H
I
A
Site 85
T1011
K
A
H
I
A
A
K
T
V
F
H
L
A
H
R
Site 86
S1061
V
D
P
N
G
K
I
S
L
Q
R
E
E
T
P
Site 87
T1067
I
S
L
Q
R
E
E
T
P
S
N
R
G
E
S
Site 88
S1069
L
Q
R
E
E
T
P
S
N
R
G
E
S
T
V
Site 89
S1074
T
P
S
N
R
G
E
S
T
V
L
S
F
V
S
Site 90
T1075
P
S
N
R
G
E
S
T
V
L
S
F
V
S
W
Site 91
S1091
T
L
S
G
P
E
Q
S
S
V
R
G
P
S
T
Site 92
S1092
L
S
G
P
E
Q
S
S
V
R
G
P
S
T
E
Site 93
S1097
Q
S
S
V
R
G
P
S
T
E
N
Q
E
A
K
Site 94
T1098
S
S
V
R
G
P
S
T
E
N
Q
E
A
K
R
Site 95
T1121
C
D
P
E
K
M
I
T
E
S
K
F
L
Q
L
Site 96
S1140
E
L
M
K
A
L
V
S
V
T
P
D
E
E
T
Site 97
T1142
M
K
A
L
V
S
V
T
P
D
E
E
T
Y
D
Site 98
Y1183
Q
T
V
R
D
H
L
Y
H
L
C
V
Q
A
Q
Site 99
T1252
G
L
H
E
L
L
K
T
N
A
A
N
I
H
S
Site 100
T1265
H
S
G
D
D
W
A
T
L
F
T
L
L
E
C
Site 101
S1298
A
P
D
A
G
A
Q
S
D
S
E
L
P
S
Y
Site 102
S1300
D
A
G
A
Q
S
D
S
E
L
P
S
Y
H
Q
Site 103
S1304
Q
S
D
S
E
L
P
S
Y
H
Q
N
D
V
S
Site 104
Y1305
S
D
S
E
L
P
S
Y
H
Q
N
D
V
S
L
Site 105
S1311
S
Y
H
Q
N
D
V
S
L
D
R
G
Y
T
S
Site 106
Y1316
D
V
S
L
D
R
G
Y
T
S
D
S
E
V
Y
Site 107
T1317
V
S
L
D
R
G
Y
T
S
D
S
E
V
Y
T
Site 108
S1318
S
L
D
R
G
Y
T
S
D
S
E
V
Y
T
D
Site 109
S1320
D
R
G
Y
T
S
D
S
E
V
Y
T
D
H
G
Site 110
Y1323
Y
T
S
D
S
E
V
Y
T
D
H
G
R
P
G
Site 111
T1324
T
S
D
S
E
V
Y
T
D
H
G
R
P
G
K
Site 112
S1335
R
P
G
K
I
H
R
S
A
T
D
A
D
V
V
Site 113
T1337
G
K
I
H
R
S
A
T
D
A
D
V
V
N
S
Site 114
S1344
T
D
A
D
V
V
N
S
G
W
L
V
V
G
K
Site 115
S1357
G
K
D
D
V
D
N
S
K
P
G
P
S
R
P
Site 116
S1362
D
N
S
K
P
G
P
S
R
P
G
P
S
P
L
Site 117
S1367
G
P
S
R
P
G
P
S
P
L
I
N
Q
Y
S
Site 118
T1386
L
D
L
G
P
H
D
T
K
S
L
L
K
C
V
Site 119
S1388
L
G
P
H
D
T
K
S
L
L
K
C
V
E
S
Site 120
T1407
V
R
D
A
A
H
I
T
P
D
N
F
E
L
C
Site 121
S1432
S
L
N
G
G
C
K
S
Q
E
K
R
G
K
S
Site 122
S1439
S
Q
E
K
R
G
K
S
H
K
Y
D
S
K
G
Site 123
Y1442
K
R
G
K
S
H
K
Y
D
S
K
G
N
R
F
Site 124
S1444
G
K
S
H
K
Y
D
S
K
G
N
R
F
K
K
Site 125
S1453
G
N
R
F
K
K
K
S
K
E
G
S
M
L
R
Site 126
S1457
K
K
K
S
K
E
G
S
M
L
R
R
P
R
T
Site 127
T1464
S
M
L
R
R
P
R
T
S
S
Q
H
A
S
R
Site 128
S1465
M
L
R
R
P
R
T
S
S
Q
H
A
S
R
G
Site 129
S1466
L
R
R
P
R
T
S
S
Q
H
A
S
R
G
G
Site 130
S1470
R
T
S
S
Q
H
A
S
R
G
G
Q
S
D
D
Site 131
S1475
H
A
S
R
G
G
Q
S
D
D
D
E
D
E
G
Site 132
S1486
E
D
E
G
V
P
A
S
Y
H
T
V
S
L
Q
Site 133
Y1487
D
E
G
V
P
A
S
Y
H
T
V
S
L
Q
V
Site 134
T1489
G
V
P
A
S
Y
H
T
V
S
L
Q
V
S
Q
Site 135
S1491
P
A
S
Y
H
T
V
S
L
Q
V
S
Q
D
L
Site 136
S1511
T
L
H
T
R
A
A
S
I
Y
S
S
W
A
E
Site 137
Y1513
H
T
R
A
A
S
I
Y
S
S
W
A
E
E
Q
Site 138
S1515
R
A
A
S
I
Y
S
S
W
A
E
E
Q
R
H
Site 139
Y1567
V
R
M
Q
A
L
T
Y
L
Q
R
A
L
L
V
Site 140
T1598
K
V
L
F
P
L
L
T
K
L
L
E
N
I
S
Site 141
S1605
T
K
L
L
E
N
I
S
P
A
D
V
G
G
M
Site 142
S1620
E
E
T
R
M
R
A
S
T
L
L
S
K
V
F
Site 143
Y1654
I
L
D
F
M
D
K
Y
M
H
A
G
S
S
D
Site 144
S1659
D
K
Y
M
H
A
G
S
S
D
L
L
S
E
A
Site 145
S1660
K
Y
M
H
A
G
S
S
D
L
L
S
E
A
I
Site 146
S1686
D
T
A
E
I
F
H
S
A
D
A
R
G
G
G
Site 147
S1695
D
A
R
G
G
G
P
S
A
L
W
E
I
T
W
Site 148
S1738
Q
G
Q
K
P
L
A
S
A
H
L
T
S
A
A
Site 149
T1742
P
L
A
S
A
H
L
T
S
A
A
G
D
T
R
Site 150
T1748
L
T
S
A
A
G
D
T
R
T
P
G
H
P
P
Site 151
T1750
S
A
A
G
D
T
R
T
P
G
H
P
P
P
P
Site 152
S1773
C
D
F
E
K
P
E
S
P
R
A
A
S
S
S
Site 153
S1778
P
E
S
P
R
A
A
S
S
S
S
P
G
S
P
Site 154
S1779
E
S
P
R
A
A
S
S
S
S
P
G
S
P
V
Site 155
S1780
S
P
R
A
A
S
S
S
S
P
G
S
P
V
A
Site 156
S1781
P
R
A
A
S
S
S
S
P
G
S
P
V
A
S
Site 157
S1784
A
S
S
S
S
P
G
S
P
V
A
S
S
P
S
Site 158
S1788
S
P
G
S
P
V
A
S
S
P
S
R
L
S
P
Site 159
S1789
P
G
S
P
V
A
S
S
P
S
R
L
S
P
T
Site 160
S1791
S
P
V
A
S
S
P
S
R
L
S
P
T
P
D
Site 161
S1794
A
S
S
P
S
R
L
S
P
T
P
D
G
P
P
Site 162
T1796
S
P
S
R
L
S
P
T
P
D
G
P
P
P
L
Site 163
T1846
S
P
V
P
L
L
A
T
P
R
P
T
D
P
I
Site 164
T1850
L
L
A
T
P
R
P
T
D
P
I
P
T
S
E
Site 165
T1855
R
P
T
D
P
I
P
T
S
E
V
N
_
_
_
Site 166
S1856
P
T
D
P
I
P
T
S
E
V
N
_
_
_
_
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation