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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
SPEN
Full Name:
Msx2-interacting protein
Alias:
KIAA0929; MINT; RBM15C; SHARP; SMART/HDAC1 associated repressor protein; SPEN homologue; Spen, transcriptional regulator
Type:
Nuclear receptor co-regulator; Transcription factor
Mass (Da):
402248
Number AA:
3664
UniProt ID:
Q96T58
International Prot ID:
IPI00045914
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005634
GO:0043226
GO:0043227
Uniprot
OncoNet
Molecular Function:
GO:0000166
GO:0003676
GO:0003677
PhosphoSite+
KinaseNET
Biological Process:
GO:0006139
GO:0006350
GO:0006355
Phosida
TranscriptoNet
STRING
Kinexus Products
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
Y29
I
I
E
H
F
K
R
Y
G
R
V
E
S
V
K
Site 2
S34
K
R
Y
G
R
V
E
S
V
K
I
L
P
K
R
Site 3
S43
K
I
L
P
K
R
G
S
E
G
G
V
A
A
F
Site 4
S58
V
D
F
V
D
I
K
S
A
Q
K
A
H
N
S
Site 5
S65
S
A
Q
K
A
H
N
S
V
N
K
M
G
D
R
Site 6
T76
M
G
D
R
D
L
R
T
D
Y
N
E
P
G
T
Site 7
Y78
D
R
D
L
R
T
D
Y
N
E
P
G
T
I
P
Site 8
S86
N
E
P
G
T
I
P
S
A
A
R
G
L
D
D
Site 9
T94
A
A
R
G
L
D
D
T
V
S
I
A
S
R
S
Site 10
S96
R
G
L
D
D
T
V
S
I
A
S
R
S
R
E
Site 11
S99
D
D
T
V
S
I
A
S
R
S
R
E
V
S
G
Site 12
S101
T
V
S
I
A
S
R
S
R
E
V
S
G
F
R
Site 13
S105
A
S
R
S
R
E
V
S
G
F
R
G
G
G
G
Site 14
Y116
G
G
G
G
G
P
A
Y
G
P
P
P
S
L
H
Site 15
S121
P
A
Y
G
P
P
P
S
L
H
A
R
E
G
R
Site 16
Y129
L
H
A
R
E
G
R
Y
E
R
R
L
D
G
A
Site 17
S137
E
R
R
L
D
G
A
S
D
N
R
E
R
A
Y
Site 18
Y144
S
D
N
R
E
R
A
Y
E
H
S
A
Y
G
H
Site 19
S147
R
E
R
A
Y
E
H
S
A
Y
G
H
H
E
R
Site 20
Y149
R
A
Y
E
H
S
A
Y
G
H
H
E
R
G
T
Site 21
T156
Y
G
H
H
E
R
G
T
G
G
F
D
R
T
R
Site 22
Y165
G
F
D
R
T
R
H
Y
D
Q
D
Y
Y
R
D
Site 23
Y169
T
R
H
Y
D
Q
D
Y
Y
R
D
P
R
E
R
Site 24
Y170
R
H
Y
D
Q
D
Y
Y
R
D
P
R
E
R
T
Site 25
T177
Y
R
D
P
R
E
R
T
L
Q
H
G
L
Y
Y
Site 26
Y183
R
T
L
Q
H
G
L
Y
Y
A
S
R
S
R
S
Site 27
Y184
T
L
Q
H
G
L
Y
Y
A
S
R
S
R
S
P
Site 28
S186
Q
H
G
L
Y
Y
A
S
R
S
R
S
P
N
R
Site 29
S188
G
L
Y
Y
A
S
R
S
R
S
P
N
R
F
D
Site 30
S190
Y
Y
A
S
R
S
R
S
P
N
R
F
D
A
H
Site 31
Y201
F
D
A
H
D
P
R
Y
E
P
R
A
R
E
Q
Site 32
T210
P
R
A
R
E
Q
F
T
L
P
S
V
V
H
R
Site 33
S213
R
E
Q
F
T
L
P
S
V
V
H
R
D
I
Y
Site 34
Y220
S
V
V
H
R
D
I
Y
R
D
D
I
T
R
E
Site 35
T225
D
I
Y
R
D
D
I
T
R
E
V
R
G
R
R
Site 36
Y237
G
R
R
P
E
R
N
Y
Q
H
S
R
S
R
S
Site 37
S240
P
E
R
N
Y
Q
H
S
R
S
R
S
P
H
S
Site 38
S242
R
N
Y
Q
H
S
R
S
R
S
P
H
S
S
Q
Site 39
S244
Y
Q
H
S
R
S
R
S
P
H
S
S
Q
S
R
Site 40
S247
S
R
S
R
S
P
H
S
S
Q
S
R
N
Q
S
Site 41
S248
R
S
R
S
P
H
S
S
Q
S
R
N
Q
S
P
Site 42
S250
R
S
P
H
S
S
Q
S
R
N
Q
S
P
Q
R
Site 43
S254
S
S
Q
S
R
N
Q
S
P
Q
R
L
A
S
Q
Site 44
S260
Q
S
P
Q
R
L
A
S
Q
A
S
R
P
T
R
Site 45
S263
Q
R
L
A
S
Q
A
S
R
P
T
R
S
P
S
Site 46
T266
A
S
Q
A
S
R
P
T
R
S
P
S
G
S
G
Site 47
S268
Q
A
S
R
P
T
R
S
P
S
G
S
G
S
R
Site 48
S270
S
R
P
T
R
S
P
S
G
S
G
S
R
S
R
Site 49
S272
P
T
R
S
P
S
G
S
G
S
R
S
R
S
S
Site 50
S274
R
S
P
S
G
S
G
S
R
S
R
S
S
S
S
Site 51
S276
P
S
G
S
G
S
R
S
R
S
S
S
S
D
S
Site 52
S278
G
S
G
S
R
S
R
S
S
S
S
D
S
I
S
Site 53
S279
S
G
S
R
S
R
S
S
S
S
D
S
I
S
S
Site 54
S280
G
S
R
S
R
S
S
S
S
D
S
I
S
S
S
Site 55
S281
S
R
S
R
S
S
S
S
D
S
I
S
S
S
S
Site 56
S283
S
R
S
S
S
S
D
S
I
S
S
S
S
S
T
Site 57
S285
S
S
S
S
D
S
I
S
S
S
S
S
T
S
S
Site 58
S286
S
S
S
D
S
I
S
S
S
S
S
T
S
S
D
Site 59
S287
S
S
D
S
I
S
S
S
S
S
T
S
S
D
S
Site 60
S288
S
D
S
I
S
S
S
S
S
T
S
S
D
S
S
Site 61
S289
D
S
I
S
S
S
S
S
T
S
S
D
S
S
D
Site 62
T290
S
I
S
S
S
S
S
T
S
S
D
S
S
D
S
Site 63
S291
I
S
S
S
S
S
T
S
S
D
S
S
D
S
S
Site 64
S292
S
S
S
S
S
T
S
S
D
S
S
D
S
S
S
Site 65
S294
S
S
S
T
S
S
D
S
S
D
S
S
S
S
S
Site 66
S295
S
S
T
S
S
D
S
S
D
S
S
S
S
S
S
Site 67
S297
T
S
S
D
S
S
D
S
S
S
S
S
S
D
D
Site 68
S298
S
S
D
S
S
D
S
S
S
S
S
S
D
D
S
Site 69
S299
S
D
S
S
D
S
S
S
S
S
S
D
D
S
P
Site 70
S300
D
S
S
D
S
S
S
S
S
S
D
D
S
P
A
Site 71
S301
S
S
D
S
S
S
S
S
S
D
D
S
P
A
R
Site 72
S302
S
D
S
S
S
S
S
S
D
D
S
P
A
R
S
Site 73
S305
S
S
S
S
S
D
D
S
P
A
R
S
V
Q
S
Site 74
S309
S
D
D
S
P
A
R
S
V
Q
S
A
A
V
P
Site 75
S312
S
P
A
R
S
V
Q
S
A
A
V
P
A
P
T
Site 76
S324
A
P
T
S
Q
L
L
S
S
L
E
K
D
E
P
Site 77
S325
P
T
S
Q
L
L
S
S
L
E
K
D
E
P
R
Site 78
S334
E
K
D
E
P
R
K
S
F
G
I
K
V
Q
N
Site 79
S346
V
Q
N
L
P
V
R
S
T
D
T
S
L
K
D
Site 80
T347
Q
N
L
P
V
R
S
T
D
T
S
L
K
D
G
Site 81
S350
P
V
R
S
T
D
T
S
L
K
D
G
L
F
H
Site 82
S367
K
K
F
G
K
V
T
S
V
Q
I
H
G
T
S
Site 83
S374
S
V
Q
I
H
G
T
S
E
E
R
Y
G
L
V
Site 84
Y378
H
G
T
S
E
E
R
Y
G
L
V
F
F
R
Q
Site 85
T394
E
D
Q
E
K
A
L
T
A
S
K
G
K
L
F
Site 86
T450
G
N
L
E
K
T
T
T
Y
H
D
L
R
N
I
Site 87
Y478
K
V
N
G
V
P
Q
Y
A
F
L
Q
Y
C
D
Site 88
Y500
I
K
K
M
D
G
E
Y
L
G
N
N
R
L
K
Site 89
S525
C
V
W
L
D
G
L
S
S
N
V
S
D
Q
Y
Site 90
S526
V
W
L
D
G
L
S
S
N
V
S
D
Q
Y
L
Site 91
S529
D
G
L
S
S
N
V
S
D
Q
Y
L
T
R
H
Site 92
Y532
S
S
N
V
S
D
Q
Y
L
T
R
H
F
C
R
Site 93
Y596
R
E
S
Q
L
A
F
Y
H
C
M
E
K
S
G
Site 94
Y610
G
Q
D
I
R
D
F
Y
E
M
L
A
E
R
R
Site 95
S623
R
R
E
E
R
R
A
S
Y
D
Y
N
Q
D
R
Site 96
Y624
R
E
E
R
R
A
S
Y
D
Y
N
Q
D
R
T
Site 97
Y626
E
R
R
A
S
Y
D
Y
N
Q
D
R
T
Y
Y
Site 98
Y632
D
Y
N
Q
D
R
T
Y
Y
E
S
V
R
T
P
Site 99
Y633
Y
N
Q
D
R
T
Y
Y
E
S
V
R
T
P
G
Site 100
S635
Q
D
R
T
Y
Y
E
S
V
R
T
P
G
T
Y
Site 101
T638
T
Y
Y
E
S
V
R
T
P
G
T
Y
P
E
D
Site 102
T641
E
S
V
R
T
P
G
T
Y
P
E
D
S
R
R
Site 103
Y642
S
V
R
T
P
G
T
Y
P
E
D
S
R
R
D
Site 104
S646
P
G
T
Y
P
E
D
S
R
R
D
Y
P
A
R
Site 105
Y650
P
E
D
S
R
R
D
Y
P
A
R
G
R
E
F
Site 106
Y658
P
A
R
G
R
E
F
Y
S
E
W
E
T
Y
Q
Site 107
S659
A
R
G
R
E
F
Y
S
E
W
E
T
Y
Q
G
Site 108
Y664
F
Y
S
E
W
E
T
Y
Q
G
D
Y
Y
E
S
Site 109
Y668
W
E
T
Y
Q
G
D
Y
Y
E
S
R
Y
Y
D
Site 110
Y669
E
T
Y
Q
G
D
Y
Y
E
S
R
Y
Y
D
D
Site 111
Y673
G
D
Y
Y
E
S
R
Y
Y
D
D
P
R
E
Y
Site 112
Y674
D
Y
Y
E
S
R
Y
Y
D
D
P
R
E
Y
R
Site 113
Y680
Y
Y
D
D
P
R
E
Y
R
D
Y
R
N
D
P
Site 114
Y683
D
P
R
E
Y
R
D
Y
R
N
D
P
Y
E
Q
Site 115
Y688
R
D
Y
R
N
D
P
Y
E
Q
D
I
R
E
Y
Site 116
Y695
Y
E
Q
D
I
R
E
Y
S
Y
R
Q
R
E
R
Site 117
S696
E
Q
D
I
R
E
Y
S
Y
R
Q
R
E
R
E
Site 118
S711
R
E
R
E
R
F
E
S
D
R
D
R
D
H
E
Site 119
S725
E
R
R
P
I
E
R
S
Q
S
P
V
H
L
R
Site 120
S727
R
P
I
E
R
S
Q
S
P
V
H
L
R
R
P
Site 121
S736
V
H
L
R
R
P
Q
S
P
G
A
S
P
S
Q
Site 122
S740
R
P
Q
S
P
G
A
S
P
S
Q
A
E
R
L
Site 123
S742
Q
S
P
G
A
S
P
S
Q
A
E
R
L
P
S
Site 124
S749
S
Q
A
E
R
L
P
S
D
S
E
R
R
L
Y
Site 125
S751
A
E
R
L
P
S
D
S
E
R
R
L
Y
S
R
Site 126
Y756
S
D
S
E
R
R
L
Y
S
R
S
S
D
R
S
Site 127
S757
D
S
E
R
R
L
Y
S
R
S
S
D
R
S
G
Site 128
S759
E
R
R
L
Y
S
R
S
S
D
R
S
G
S
C
Site 129
S760
R
R
L
Y
S
R
S
S
D
R
S
G
S
C
S
Site 130
S763
Y
S
R
S
S
D
R
S
G
S
C
S
S
L
S
Site 131
S765
R
S
S
D
R
S
G
S
C
S
S
L
S
P
P
Site 132
S767
S
D
R
S
G
S
C
S
S
L
S
P
P
R
Y
Site 133
S768
D
R
S
G
S
C
S
S
L
S
P
P
R
Y
E
Site 134
S770
S
G
S
C
S
S
L
S
P
P
R
Y
E
K
L
Site 135
Y774
S
S
L
S
P
P
R
Y
E
K
L
D
K
S
R
Site 136
S780
R
Y
E
K
L
D
K
S
R
L
E
R
Y
T
K
Site 137
Y785
D
K
S
R
L
E
R
Y
T
K
N
E
K
T
D
Site 138
T786
K
S
R
L
E
R
Y
T
K
N
E
K
T
D
K
Site 139
T791
R
Y
T
K
N
E
K
T
D
K
E
R
T
F
D
Site 140
T796
E
K
T
D
K
E
R
T
F
D
P
E
R
V
E
Site 141
T819
E
K
V
E
K
D
K
T
D
K
Q
K
R
K
G
Site 142
S830
K
R
K
G
K
V
H
S
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Y3306
S
G
E
L
F
Q
E
Y
R
Y
G
D
I
R
T
Site 447
Y3308
E
L
F
Q
E
Y
R
Y
G
D
I
R
T
Y
H
Site 448
T3313
Y
R
Y
G
D
I
R
T
Y
H
P
P
A
Q
L
Site 449
Y3314
R
Y
G
D
I
R
T
Y
H
P
P
A
Q
L
T
Site 450
T3321
Y
H
P
P
A
Q
L
T
H
T
Q
F
P
A
A
Site 451
T3323
P
P
A
Q
L
T
H
T
Q
F
P
A
A
S
S
Site 452
S3329
H
T
Q
F
P
A
A
S
S
V
G
L
P
S
R
Site 453
S3335
A
S
S
V
G
L
P
S
R
T
K
T
A
A
Q
Site 454
T3339
G
L
P
S
R
T
K
T
A
A
Q
G
P
P
P
Site 455
S3359
Q
P
P
Q
P
V
Q
S
T
Q
P
A
Q
P
A
Site 456
S3372
P
A
P
P
C
P
P
S
Q
L
G
Q
P
G
Q
Site 457
S3382
G
Q
P
G
Q
P
P
S
S
K
M
P
Q
V
S
Site 458
S3383
Q
P
G
Q
P
P
S
S
K
M
P
Q
V
S
Q
Site 459
S3389
S
S
K
M
P
Q
V
S
Q
E
A
K
G
T
Q
Site 460
T3395
V
S
Q
E
A
K
G
T
Q
T
G
V
E
Q
P
Site 461
T3426
V
Q
R
A
Q
A
E
T
G
P
T
S
F
P
S
Site 462
T3429
A
Q
A
E
T
G
P
T
S
F
P
S
P
V
S
Site 463
S3430
Q
A
E
T
G
P
T
S
F
P
S
P
V
S
V
Site 464
S3433
T
G
P
T
S
F
P
S
P
V
S
V
S
M
K
Site 465
S3436
T
S
F
P
S
P
V
S
V
S
M
K
P
D
L
Site 466
S3446
M
K
P
D
L
P
V
S
L
P
T
Q
T
A
P
Site 467
T3462
Q
P
L
F
V
P
T
T
S
G
P
S
T
P
P
Site 468
S3463
P
L
F
V
P
T
T
S
G
P
S
T
P
P
G
Site 469
S3466
V
P
T
T
S
G
P
S
T
P
P
G
L
V
L
Site 470
T3467
P
T
T
S
G
P
S
T
P
P
G
L
V
L
P
Site 471
T3476
P
G
L
V
L
P
H
T
E
F
Q
P
A
P
K
Site 472
S3486
Q
P
A
P
K
Q
D
S
S
P
H
L
T
S
Q
Site 473
S3487
P
A
P
K
Q
D
S
S
P
H
L
T
S
Q
R
Site 474
T3491
Q
D
S
S
P
H
L
T
S
Q
R
P
V
D
M
Site 475
S3492
D
S
S
P
H
L
T
S
Q
R
P
V
D
M
V
Site 476
S3537
N
N
V
L
A
H
R
S
L
P
L
S
E
G
G
Site 477
S3541
A
H
R
S
L
P
L
S
E
G
G
P
P
L
R
Site 478
T3558
Q
R
M
R
L
E
A
T
Q
L
E
G
V
A
R
Site 479
T3568
E
G
V
A
R
R
M
T
V
E
T
D
Y
C
L
Site 480
S3590
R
D
Q
E
D
V
V
S
Q
T
E
S
L
K
A
Site 481
S3619
I
N
V
P
N
P
G
S
N
Q
P
A
Y
V
L
Site 482
Y3624
P
G
S
N
Q
P
A
Y
V
L
Q
I
F
P
P
Site 483
S3635
I
F
P
P
C
E
F
S
E
S
H
L
S
R
L
Site 484
S3637
P
P
C
E
F
S
E
S
H
L
S
R
L
A
P
Site 485
S3640
E
F
S
E
S
H
L
S
R
L
A
P
D
L
L
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation