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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
USP25
Full Name:
Ubiquitin carboxyl-terminal hydrolase 25
Alias:
Deubiquitinating enzyme 25; Ubiquitin specific peptidase 25; Ubiquitin specific protease 25; Ubiquitin thiolesterase 25; Ubiquitin-specific processing protease 25; UBP25; USP on chromosome 21; USP21
Type:
EC 3.1.2.15; Ubiquitin conjugating system; Protease
Mass (Da):
125750
Number AA:
1087
UniProt ID:
Q9UHP3
International Prot ID:
IPI00300439
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
Uniprot
OncoNet
Molecular Function:
GO:0004221
GO:0004843
PhosphoSite+
KinaseNET
Biological Process:
GO:0006464
GO:0006511
Phosida
TranscriptoNet
STRING
Kinexus Products
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
T19
A
A
Q
K
H
Q
Q
T
F
L
N
Q
L
R
E
Site 2
T28
L
N
Q
L
R
E
I
T
G
I
N
D
T
Q
I
Site 3
T55
E
L
A
V
A
F
L
T
A
K
N
A
K
T
P
Site 4
T61
L
T
A
K
N
A
K
T
P
Q
Q
E
E
T
T
Site 5
Y69
P
Q
Q
E
E
T
T
Y
Y
Q
T
A
L
P
G
Site 6
Y70
Q
Q
E
E
T
T
Y
Y
Q
T
A
L
P
G
N
Site 7
Y80
A
L
P
G
N
D
R
Y
I
S
V
G
S
Q
A
Site 8
S82
P
G
N
D
R
Y
I
S
V
G
S
Q
A
D
T
Site 9
S85
D
R
Y
I
S
V
G
S
Q
A
D
T
N
V
I
Site 10
T89
S
V
G
S
Q
A
D
T
N
V
I
D
L
T
G
Site 11
T95
D
T
N
V
I
D
L
T
G
D
D
K
D
D
L
Site 12
S109
L
Q
R
A
I
A
L
S
L
A
E
S
N
R
A
Site 13
S113
I
A
L
S
L
A
E
S
N
R
A
F
R
E
T
Site 14
T120
S
N
R
A
F
R
E
T
G
I
T
D
E
E
Q
Site 15
T123
A
F
R
E
T
G
I
T
D
E
E
Q
A
I
S
Site 16
S130
T
D
E
E
Q
A
I
S
R
V
L
E
A
S
I
Site 17
T147
N
K
A
C
L
K
R
T
P
T
E
V
W
R
D
Site 18
T149
A
C
L
K
R
T
P
T
E
V
W
R
D
S
R
Site 19
S155
P
T
E
V
W
R
D
S
R
N
P
Y
D
R
K
Site 20
Y159
W
R
D
S
R
N
P
Y
D
R
K
R
Q
D
K
Site 21
S186
W
F
S
A
V
I
Q
S
L
F
N
L
L
E
F
Site 22
Y200
F
R
R
L
V
L
N
Y
K
P
P
S
N
A
Q
Site 23
S204
V
L
N
Y
K
P
P
S
N
A
Q
D
L
P
R
Site 24
Y239
L
V
G
T
K
R
K
Y
V
D
P
S
R
A
V
Site 25
S255
I
L
K
D
A
F
K
S
N
D
S
Q
Q
Q
D
Site 26
S258
D
A
F
K
S
N
D
S
Q
Q
Q
D
V
S
E
Site 27
S264
D
S
Q
Q
Q
D
V
S
E
F
T
H
K
L
L
Site 28
T317
E
G
K
K
F
E
N
T
E
M
F
G
Q
Y
P
Site 29
Y323
N
T
E
M
F
G
Q
Y
P
L
Q
V
N
G
F
Site 30
S348
M
I
E
G
E
I
E
S
L
H
S
E
N
S
G
Site 31
S351
G
E
I
E
S
L
H
S
E
N
S
G
K
S
G
Site 32
S354
E
S
L
H
S
E
N
S
G
K
S
G
Q
E
H
Site 33
T364
S
G
Q
E
H
W
F
T
E
L
P
P
V
L
T
Site 34
T371
T
E
L
P
P
V
L
T
F
E
L
S
R
F
E
Site 35
S375
P
V
L
T
F
E
L
S
R
F
E
F
N
Q
A
Site 36
Y400
L
E
F
P
Q
V
L
Y
L
D
R
Y
M
H
R
Site 37
Y404
Q
V
L
Y
L
D
R
Y
M
H
R
N
R
E
I
Site 38
T412
M
H
R
N
R
E
I
T
R
I
K
R
E
E
I
Site 39
Y425
E
I
K
R
L
K
D
Y
L
T
V
L
Q
Q
R
Site 40
T427
K
R
L
K
D
Y
L
T
V
L
Q
Q
R
L
E
Site 41
Y436
L
Q
Q
R
L
E
R
Y
L
S
Y
G
S
G
P
Site 42
S438
Q
R
L
E
R
Y
L
S
Y
G
S
G
P
K
R
Site 43
S461
Y
A
L
E
F
A
S
S
K
P
V
C
T
S
P
Site 44
S467
S
S
K
P
V
C
T
S
P
V
D
D
I
D
A
Site 45
S475
P
V
D
D
I
D
A
S
S
P
P
S
G
S
I
Site 46
S476
V
D
D
I
D
A
S
S
P
P
S
G
S
I
P
Site 47
S479
I
D
A
S
S
P
P
S
G
S
I
P
S
Q
T
Site 48
S481
A
S
S
P
P
S
G
S
I
P
S
Q
T
L
P
Site 49
S484
P
P
S
G
S
I
P
S
Q
T
L
P
S
T
T
Site 50
T486
S
G
S
I
P
S
Q
T
L
P
S
T
T
E
Q
Site 51
T490
P
S
Q
T
L
P
S
T
T
E
Q
Q
G
A
L
Site 52
S498
T
E
Q
Q
G
A
L
S
S
E
L
P
S
T
S
Site 53
S499
E
Q
Q
G
A
L
S
S
E
L
P
S
T
S
P
Site 54
S503
A
L
S
S
E
L
P
S
T
S
P
S
S
V
A
Site 55
T504
L
S
S
E
L
P
S
T
S
P
S
S
V
A
A
Site 56
S505
S
S
E
L
P
S
T
S
P
S
S
V
A
A
I
Site 57
S507
E
L
P
S
T
S
P
S
S
V
A
A
I
S
S
Site 58
S508
L
P
S
T
S
P
S
S
V
A
A
I
S
S
R
Site 59
S513
P
S
S
V
A
A
I
S
S
R
S
V
I
H
K
Site 60
S514
S
S
V
A
A
I
S
S
R
S
V
I
H
K
P
Site 61
T523
S
V
I
H
K
P
F
T
Q
S
R
I
P
P
D
Site 62
T541
H
P
A
P
R
H
I
T
E
E
E
L
S
V
L
Site 63
S546
H
I
T
E
E
E
L
S
V
L
E
S
C
L
H
Site 64
S550
E
E
L
S
V
L
E
S
C
L
H
R
W
R
T
Site 65
T557
S
C
L
H
R
W
R
T
E
I
E
N
D
T
R
Site 66
S569
D
T
R
D
L
Q
E
S
I
S
R
I
H
R
T
Site 67
S571
R
D
L
Q
E
S
I
S
R
I
H
R
T
I
E
Site 68
Y581
H
R
T
I
E
L
M
Y
S
D
K
S
M
I
Q
Site 69
S582
R
T
I
E
L
M
Y
S
D
K
S
M
I
Q
V
Site 70
S585
E
L
M
Y
S
D
K
S
M
I
Q
V
P
Y
R
Site 71
Y623
R
E
S
R
W
M
K
Y
N
D
I
A
V
T
K
Site 72
S631
N
D
I
A
V
T
K
S
S
W
E
E
L
V
R
Site 73
S632
D
I
A
V
T
K
S
S
W
E
E
L
V
R
D
Site 74
S640
W
E
E
L
V
R
D
S
F
G
G
Y
R
N
A
Site 75
Y644
V
R
D
S
F
G
G
Y
R
N
A
S
A
Y
C
Site 76
Y650
G
Y
R
N
A
S
A
Y
C
L
M
Y
I
N
D
Site 77
T680
Q
P
L
V
G
I
E
T
L
P
P
D
L
R
D
Site 78
S725
A
S
Q
K
L
R
E
S
E
T
S
V
T
T
A
Site 79
S728
K
L
R
E
S
E
T
S
V
T
T
A
Q
A
A
Site 80
T730
R
E
S
E
T
S
V
T
T
A
Q
A
A
G
D
Site 81
Y740
Q
A
A
G
D
P
E
Y
L
E
Q
P
S
R
S
Site 82
S745
P
E
Y
L
E
Q
P
S
R
S
D
F
S
K
H
Site 83
S747
Y
L
E
Q
P
S
R
S
D
F
S
K
H
L
K
Site 84
T762
E
E
T
I
Q
I
I
T
K
A
S
H
E
H
E
Site 85
S772
S
H
E
H
E
D
K
S
P
E
T
V
L
Q
S
Site 86
T775
H
E
D
K
S
P
E
T
V
L
Q
S
I
M
M
Site 87
S798
M
A
I
G
K
S
R
S
V
Y
D
R
C
G
P
Site 88
Y800
I
G
K
S
R
S
V
Y
D
R
C
G
P
E
A
Site 89
T828
V
K
L
A
Q
E
D
T
P
P
E
T
D
Y
R
Site 90
T832
Q
E
D
T
P
P
E
T
D
Y
R
L
H
H
V
Site 91
Y834
D
T
P
P
E
T
D
Y
R
L
H
H
V
V
V
Site 92
T856
P
K
K
I
I
E
K
T
L
L
E
Q
F
G
D
Site 93
S867
Q
F
G
D
R
N
L
S
F
D
E
R
C
H
N
Site 94
Y896
E
E
V
N
L
E
E
Y
E
E
W
H
Q
D
Y
Site 95
Y903
Y
E
E
W
H
Q
D
Y
R
K
F
R
E
T
T
Site 96
T910
Y
R
K
F
R
E
T
T
M
Y
L
I
I
G
L
Site 97
Y912
K
F
R
E
T
T
M
Y
L
I
I
G
L
E
N
Site 98
S924
L
E
N
F
Q
R
E
S
Y
I
D
S
L
L
F
Site 99
Y925
E
N
F
Q
R
E
S
Y
I
D
S
L
L
F
L
Site 100
Y948
E
L
L
S
K
G
L
Y
R
G
H
D
E
E
L
Site 101
S957
G
H
D
E
E
L
I
S
H
Y
R
R
E
C
L
Site 102
Y959
D
E
E
L
I
S
H
Y
R
R
E
C
L
L
K
Site 103
S977
Q
A
A
E
L
F
E
S
G
E
D
R
E
V
N
Site 104
S1023
D
M
R
N
R
W
C
S
Y
L
G
Q
E
M
E
Site 105
T1038
P
H
L
Q
E
K
L
T
D
F
L
P
K
L
L
Site 106
S1048
L
P
K
L
L
D
C
S
M
E
I
K
S
F
H
Site 107
S1053
D
C
S
M
E
I
K
S
F
H
E
P
P
K
L
Site 108
S1062
H
E
P
P
K
L
P
S
Y
S
T
H
E
L
C
Site 109
Y1063
E
P
P
K
L
P
S
Y
S
T
H
E
L
C
E
Site 110
S1064
P
P
K
L
P
S
Y
S
T
H
E
L
C
E
R
Site 111
S1078
R
F
A
R
I
M
L
S
L
S
R
T
P
A
D
Site 112
S1080
A
R
I
M
L
S
L
S
R
T
P
A
D
G
R
Site 113
T1082
I
M
L
S
L
S
R
T
P
A
D
G
R
_
_
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation