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Updated November 2019
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Protein Info
Short Name:
TNIK
Full Name:
TRAF2 and NCK-interacting protein kinase
Alias:
EC 2.7.11.1; KIAA0551; TRAF2 and NCK interacting kinase
Type:
EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor);
STE
group; STE20 family; MSN subfamily
Mass (Da):
154943
Number AA:
1360
UniProt ID:
Q9UKE5
International Prot ID:
IPI00145805
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
Uniprot
OncoNet
Molecular Function:
GO:0005524
GO:0005515
GO:0004674
PhosphoSite+
KinaseNET
Biological Process:
GO:0007254
GO:0006468
GO:0006950
Phosida
TranscriptoNet
STRING
Kinexus Products
TRAF2 and NCK-interacting kinase pan-specific antibody AB-NK301-1#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK301-1
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S5
_
_
_
M
A
S
D
S
P
A
R
S
L
D
E
Site 2
S9
A
S
D
S
P
A
R
S
L
D
E
I
D
L
S
Site 3
S16
S
L
D
E
I
D
L
S
A
L
R
D
P
A
G
Site 4
Y36
E
L
V
G
N
G
T
Y
G
Q
V
Y
K
G
R
Site 5
Y40
N
G
T
Y
G
Q
V
Y
K
G
R
H
V
K
T
Site 6
T59
A
I
K
V
M
D
V
T
G
D
E
E
E
E
I
Site 7
Y76
E
I
N
M
L
K
K
Y
S
H
H
R
N
I
A
Site 8
Y85
H
H
R
N
I
A
T
Y
Y
G
A
F
I
K
K
Site 9
Y86
H
R
N
I
A
T
Y
Y
G
A
F
I
K
K
N
Site 10
Y132
L
K
E
E
W
I
A
Y
I
C
R
E
I
L
R
Site 11
S142
R
E
I
L
R
G
L
S
H
L
H
Q
H
K
V
Site 12
T162
K
G
Q
N
V
L
L
T
E
N
A
E
V
K
L
Site 13
S175
K
L
V
D
F
G
V
S
A
Q
L
D
R
T
V
Site 14
T181
V
S
A
Q
L
D
R
T
V
G
R
R
N
T
F
Site 15
T187
R
T
V
G
R
R
N
T
F
I
G
T
P
Y
W
Site 16
T191
R
R
N
T
F
I
G
T
P
Y
W
M
A
P
E
Site 17
Y193
N
T
F
I
G
T
P
Y
W
M
A
P
E
V
I
Site 18
T209
C
D
E
N
P
D
A
T
Y
D
F
K
S
D
L
Site 19
Y210
D
E
N
P
D
A
T
Y
D
F
K
S
D
L
W
Site 20
S214
D
A
T
Y
D
F
K
S
D
L
W
S
L
G
I
Site 21
S255
N
P
A
P
R
L
K
S
K
K
W
S
K
K
F
Site 22
S259
R
L
K
S
K
K
W
S
K
K
F
Q
S
F
I
Site 23
S264
K
W
S
K
K
F
Q
S
F
I
E
S
C
L
V
Site 24
S275
S
C
L
V
K
N
H
S
Q
R
P
A
T
E
Q
Site 25
T280
N
H
S
Q
R
P
A
T
E
Q
L
M
K
H
P
Site 26
T309
L
K
D
H
I
D
R
T
K
K
K
R
G
E
K
Site 27
T319
K
R
G
E
K
D
E
T
E
Y
E
Y
S
G
S
Site 28
Y321
G
E
K
D
E
T
E
Y
E
Y
S
G
S
E
E
Site 29
Y323
K
D
E
T
E
Y
E
Y
S
G
S
E
E
E
E
Site 30
S324
D
E
T
E
Y
E
Y
S
G
S
E
E
E
E
E
Site 31
S326
T
E
Y
E
Y
S
G
S
E
E
E
E
E
E
N
Site 32
S335
E
E
E
E
E
N
D
S
G
E
P
S
S
I
L
Site 33
S339
E
N
D
S
G
E
P
S
S
I
L
N
L
P
G
Site 34
S340
N
D
S
G
E
P
S
S
I
L
N
L
P
G
E
Site 35
S348
I
L
N
L
P
G
E
S
T
L
R
R
D
F
L
Site 36
T349
L
N
L
P
G
E
S
T
L
R
R
D
F
L
R
Site 37
Y427
E
R
E
Q
R
R
H
Y
E
E
Q
M
R
R
E
Site 38
Y446
R
A
E
H
E
Q
E
Y
I
R
R
Q
L
E
E
Site 39
Y475
E
Q
A
L
L
L
E
Y
K
R
K
Q
L
E
E
Site 40
Y499
Q
L
K
Q
E
R
D
Y
L
V
S
L
Q
H
Q
Site 41
S502
Q
E
R
D
Y
L
V
S
L
Q
H
Q
R
Q
E
Site 42
Y519
P
V
E
K
K
P
L
Y
H
Y
K
E
G
M
S
Site 43
Y521
E
K
K
P
L
Y
H
Y
K
E
G
M
S
P
S
Site 44
S526
Y
H
Y
K
E
G
M
S
P
S
E
K
P
A
W
Site 45
S528
Y
K
E
G
M
S
P
S
E
K
P
A
W
A
K
Site 46
S547
R
S
R
L
N
R
Q
S
S
P
A
M
P
H
K
Site 47
S548
S
R
L
N
R
Q
S
S
P
A
M
P
H
K
V
Site 48
S560
H
K
V
A
N
R
I
S
D
P
N
L
P
P
R
Site 49
S568
D
P
N
L
P
P
R
S
E
S
F
S
I
S
G
Site 50
S570
N
L
P
P
R
S
E
S
F
S
I
S
G
V
Q
Site 51
S572
P
P
R
S
E
S
F
S
I
S
G
V
Q
P
A
Site 52
S574
R
S
E
S
F
S
I
S
G
V
Q
P
A
R
T
Site 53
T581
S
G
V
Q
P
A
R
T
P
P
M
L
R
P
V
Site 54
T606
K
S
Q
G
P
A
L
T
A
S
Q
S
V
H
E
Site 55
S608
Q
G
P
A
L
T
A
S
Q
S
V
H
E
Q
P
Site 56
S610
P
A
L
T
A
S
Q
S
V
H
E
Q
P
T
K
Site 57
S620
E
Q
P
T
K
G
L
S
G
F
Q
E
A
L
N
Site 58
S630
Q
E
A
L
N
V
T
S
H
R
V
E
M
P
R
Site 59
S640
V
E
M
P
R
Q
N
S
D
P
T
S
E
N
P
Site 60
T643
P
R
Q
N
S
D
P
T
S
E
N
P
P
L
P
Site 61
S644
R
Q
N
S
D
P
T
S
E
N
P
P
L
P
T
Site 62
S659
R
I
E
K
F
D
R
S
S
W
L
R
Q
E
E
Site 63
S660
I
E
K
F
D
R
S
S
W
L
R
Q
E
E
D
Site 64
T676
P
P
K
V
P
Q
R
T
T
S
I
S
P
A
L
Site 65
T677
P
K
V
P
Q
R
T
T
S
I
S
P
A
L
A
Site 66
S678
K
V
P
Q
R
T
T
S
I
S
P
A
L
A
R
Site 67
S680
P
Q
R
T
T
S
I
S
P
A
L
A
R
K
N
Site 68
S688
P
A
L
A
R
K
N
S
P
G
N
G
S
A
L
Site 69
S701
A
L
G
P
R
L
G
S
Q
P
I
R
A
S
N
Site 70
S707
G
S
Q
P
I
R
A
S
N
P
D
L
R
R
T
Site 71
T714
S
N
P
D
L
R
R
T
E
P
I
L
E
S
P
Site 72
S720
R
T
E
P
I
L
E
S
P
L
Q
R
T
S
S
Site 73
T725
L
E
S
P
L
Q
R
T
S
S
G
S
S
S
S
Site 74
S726
E
S
P
L
Q
R
T
S
S
G
S
S
S
S
S
Site 75
S727
S
P
L
Q
R
T
S
S
G
S
S
S
S
S
S
Site 76
S729
L
Q
R
T
S
S
G
S
S
S
S
S
S
T
P
Site 77
S730
Q
R
T
S
S
G
S
S
S
S
S
S
T
P
S
Site 78
S731
R
T
S
S
G
S
S
S
S
S
S
T
P
S
S
Site 79
S732
T
S
S
G
S
S
S
S
S
S
T
P
S
S
Q
Site 80
S733
S
S
G
S
S
S
S
S
S
T
P
S
S
Q
P
Site 81
S734
S
G
S
S
S
S
S
S
T
P
S
S
Q
P
S
Site 82
T735
G
S
S
S
S
S
S
T
P
S
S
Q
P
S
S
Site 83
S737
S
S
S
S
S
T
P
S
S
Q
P
S
S
Q
G
Site 84
S738
S
S
S
S
T
P
S
S
Q
P
S
S
Q
G
G
Site 85
S741
S
T
P
S
S
Q
P
S
S
Q
G
G
S
Q
P
Site 86
S742
T
P
S
S
Q
P
S
S
Q
G
G
S
Q
P
G
Site 87
S746
Q
P
S
S
Q
G
G
S
Q
P
G
S
Q
A
G
Site 88
S750
Q
G
G
S
Q
P
G
S
Q
A
G
S
S
E
R
Site 89
S754
Q
P
G
S
Q
A
G
S
S
E
R
T
R
V
R
Site 90
S755
P
G
S
Q
A
G
S
S
E
R
T
R
V
R
A
Site 91
S764
R
T
R
V
R
A
N
S
K
S
E
G
S
P
V
Site 92
S766
R
V
R
A
N
S
K
S
E
G
S
P
V
L
P
Site 93
S769
A
N
S
K
S
E
G
S
P
V
L
P
H
E
P
Site 94
T788
P
E
E
S
R
D
I
T
R
P
S
R
P
A
S
Site 95
S791
S
R
D
I
T
R
P
S
R
P
A
S
Y
K
K
Site 96
S795
T
R
P
S
R
P
A
S
Y
K
K
A
I
D
E
Site 97
T805
K
A
I
D
E
D
L
T
A
L
A
K
E
L
R
Site 98
T819
R
E
L
R
I
E
E
T
N
R
P
M
K
K
V
Site 99
T827
N
R
P
M
K
K
V
T
D
Y
S
S
S
S
E
Site 100
Y829
P
M
K
K
V
T
D
Y
S
S
S
S
E
E
S
Site 101
S830
M
K
K
V
T
D
Y
S
S
S
S
E
E
S
E
Site 102
S831
K
K
V
T
D
Y
S
S
S
S
E
E
S
E
S
Site 103
S832
K
V
T
D
Y
S
S
S
S
E
E
S
E
S
S
Site 104
S833
V
T
D
Y
S
S
S
S
E
E
S
E
S
S
E
Site 105
S836
Y
S
S
S
S
E
E
S
E
S
S
E
E
E
E
Site 106
S838
S
S
S
E
E
S
E
S
S
E
E
E
E
E
D
Site 107
S839
S
S
E
E
S
E
S
S
E
E
E
E
E
D
G
Site 108
S848
E
E
E
E
D
G
E
S
E
T
H
D
G
T
V
Site 109
T854
E
S
E
T
H
D
G
T
V
A
V
S
D
I
P
Site 110
T866
D
I
P
R
L
I
P
T
G
A
P
G
S
N
E
Site 111
S871
I
P
T
G
A
P
G
S
N
E
Q
Y
N
V
G
Site 112
Y875
A
P
G
S
N
E
Q
Y
N
V
G
M
V
G
T
Site 113
S888
G
T
H
G
L
E
T
S
H
A
D
S
F
S
G
Site 114
S892
L
E
T
S
H
A
D
S
F
S
G
S
I
S
R
Site 115
S894
T
S
H
A
D
S
F
S
G
S
I
S
R
E
G
Site 116
S896
H
A
D
S
F
S
G
S
I
S
R
E
G
T
L
Site 117
S898
D
S
F
S
G
S
I
S
R
E
G
T
L
M
I
Site 118
T902
G
S
I
S
R
E
G
T
L
M
I
R
E
T
S
Site 119
T908
G
T
L
M
I
R
E
T
S
G
E
K
K
R
S
Site 120
S909
T
L
M
I
R
E
T
S
G
E
K
K
R
S
G
Site 121
S915
T
S
G
E
K
K
R
S
G
H
S
D
S
N
G
Site 122
S918
E
K
K
R
S
G
H
S
D
S
N
G
F
A
G
Site 123
S920
K
R
S
G
H
S
D
S
N
G
F
A
G
H
I
Site 124
S936
L
P
D
L
V
Q
Q
S
H
S
P
A
G
T
P
Site 125
S938
D
L
V
Q
Q
S
H
S
P
A
G
T
P
T
E
Site 126
T942
Q
S
H
S
P
A
G
T
P
T
E
G
L
G
R
Site 127
S951
T
E
G
L
G
R
V
S
T
H
S
Q
E
M
D
Site 128
T952
E
G
L
G
R
V
S
T
H
S
Q
E
M
D
S
Site 129
S954
L
G
R
V
S
T
H
S
Q
E
M
D
S
G
T
Site 130
S959
T
H
S
Q
E
M
D
S
G
T
E
Y
G
M
G
Site 131
T961
S
Q
E
M
D
S
G
T
E
Y
G
M
G
S
S
Site 132
Y963
E
M
D
S
G
T
E
Y
G
M
G
S
S
T
K
Site 133
S968
T
E
Y
G
M
G
S
S
T
K
A
S
F
T
P
Site 134
T969
E
Y
G
M
G
S
S
T
K
A
S
F
T
P
F
Site 135
S972
M
G
S
S
T
K
A
S
F
T
P
F
V
D
P
Site 136
T974
S
S
T
K
A
S
F
T
P
F
V
D
P
R
V
Site 137
Y982
P
F
V
D
P
R
V
Y
Q
T
S
P
T
D
E
Site 138
S985
D
P
R
V
Y
Q
T
S
P
T
D
E
D
E
E
Site 139
T987
R
V
Y
Q
T
S
P
T
D
E
D
E
E
D
E
Site 140
S996
E
D
E
E
D
E
E
S
S
A
A
A
L
F
T
Site 141
S1021
L
N
E
A
R
K
I
S
V
V
N
V
N
P
T
Site 142
T1028
S
V
V
N
V
N
P
T
N
I
R
P
H
S
D
Site 143
S1034
P
T
N
I
R
P
H
S
D
T
P
E
I
R
K
Site 144
T1036
N
I
R
P
H
S
D
T
P
E
I
R
K
Y
K
Site 145
Y1042
D
T
P
E
I
R
K
Y
K
K
R
F
N
S
E
Site 146
S1048
K
Y
K
K
R
F
N
S
E
I
L
C
A
A
L
Site 147
Y1080
R
S
G
Q
G
K
V
Y
N
L
I
N
R
R
R
Site 148
T1102
E
G
L
N
V
L
V
T
I
S
G
K
K
N
K
Site 149
Y1113
K
K
N
K
L
R
V
Y
Y
L
S
W
L
R
N
Site 150
Y1114
K
N
K
L
R
V
Y
Y
L
S
W
L
R
N
R
Site 151
S1116
K
L
R
V
Y
Y
L
S
W
L
R
N
R
I
L
Site 152
T1137
E
K
K
Q
G
W
I
T
V
G
D
L
E
G
C
Site 153
Y1147
D
L
E
G
C
I
H
Y
K
V
V
K
Y
E
R
Site 154
T1198
K
P
L
L
V
D
L
T
V
E
E
G
Q
R
L
Site 155
Y1226
D
V
D
S
G
N
S
Y
D
I
Y
I
P
S
H
Site 156
Y1229
S
G
N
S
Y
D
I
Y
I
P
S
H
I
Q
G
Site 157
Y1264
C
Y
E
D
E
G
V
Y
V
N
T
Y
G
R
I
Site 158
Y1268
E
G
V
Y
V
N
T
Y
G
R
I
T
K
D
V
Site 159
S1306
E
K
A
I
E
I
R
S
V
E
T
G
H
L
D
Site 160
S1338
N
D
K
V
F
F
A
S
V
R
S
G
G
S
S
Site 161
T1351
S
S
Q
V
F
F
M
T
L
N
R
N
S
M
M
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation